Cancer Cell

NCI-Designated Cancer Center

Shared Resources


Technology supporting the three programs

NCI-CCThere are currently 10 Cancer Center Shared Resources that provide access to advanced instrumentation and specialized services for our scientists, as well other nonprofit and for-profit investigators in cancer research. Each Core is equipped with advanced technology and staffed by technical experts in the field.

 

Brochure

 

Animal Resources

Animal Facility - Vivarium with full-service husbandry (colony management, breeding, weaning, tail samples), , and procedures, including injections, bleeds, tumor measurements, and surgeries

Animal Imaging & Analysis - Live animal imaging utilizing luminescence, fluorescence, ultrasound, and X-rays. Hematology (CBC) and blood chemistry analysis

Tumor Analysis - Supports xenograft tumor studies including PDX, testing of anti-tumor compound efficacy, deriving cell cultures from PDX models for HTS analysis
 

Cell Imaging and Histology

Cell Imaging - 10 microscopes: bright field, fluorescence, and three confocal (including multi-photon), integrated with advanced image capture systems and analysis software.  Live cell imaging, FRET, laser TIRF

Histology - FFixed/frozen sections, automated staining for H&E and IHC, laser capture, high resolution slide scanning (bright field and fluorescence), assistance with tissue acquisition, including TMAs 
 

Structural Biology

Nuclear Magnetic Resonance - Infrastructure includes, two 600 MHz instruments with cryoprobes, two 500 MHz instruments - one with wide bore probe for solid state analysis, and a highly automated 400 MHz instrument.

Crystallography - Robotics and imaging incubators for crystallization trials, X-ray generator with 2 detectors

Protein Analysis - Includes micro-ITC, microscale thermophoresis, DSC, and Analytical Ultracentrifugation 
 

Flow Cytometry

Flow Cytometry - Core staff operate two BD cell sorters with up to 13 colors, which are housed in biosafety enclosures.  There are also five analytical cytometers with up to 14 colors, and an Amnis imaging flow cytometer
 

Genomics

DNA Analysis - Next-gen sequencing analysis  including library prep, sequencing (Illumina NextSeq 500), and data analysis. Full-service Q-PCR, NanoString nCounter, STR cell line verification, shared analytical equipment 
 

Proteomics

Proteomics - Analysis from simple protein IDs to characterizing proteome-wide PTMs. Automated sample prep, advanced Thermo LC-MS systems include two Fusion Lumos Tribrids, Velos Elite, QExactive plus, and Quantiva
 

Chemical Library Screening (Part of the Prebys Center)

Assay Development - Assists in designing, developing, and and implementing HTS assays in 384/1536 well formats, utilizing a wide variety of assay types, and also supports follow-up SAR studies

Chemical Library & High-Throughput Screening - Broad and focused libraries of compounds, including cancer-targeted drugs/compounds.  Biochemical and cell-based HTS utilizing extensive robotics and assay instrumentation

High Contrast Screening - Develops, performs, and analyzes high throughput microscopy-based assays on fixed or live cells, utilizing a PE Opera Phenix with integrated robotics or several other HCS systems

Medicinal Chemistry - Custom compound synthesis, library design/synthesis, guidance in hit to lead

Cheminformatics – Supports HTS - compound tracking/availability, data processing, SAR follow-up 
 

Bioinformatics

Bioinformatics – Informatics, statistical, and pathway analysis of large ‘omics datasets, data mining, systems biology, investigator training, shared tools including Ingenuity Pathways, NextBio, GeneGo Metacore
 

Functional Genomics

Functional Genomics - siRNA/shRNA/miRNA/cDNA/CRISPR libraries, HTS assay development and screening, hit follow-up, CRISPR-engineered cell lines, production of viral vectors (lentivirus, retrovirus, adenovirus, AAV)
 

Cancer Metabolism

Cancer Metabolism - Measurements of metabolic flux (GC-MS), major metabolites, and cellular respiration
 

Additional External Shared Resources

NCI Cancer Centers Council (C3) Shared Resources at nearby Cancer Centers
Lake Nona Metabolomics Core - The metabolomics core at Lake Nona performs targeted metabolomics of acylcarnitines, amino acids, organic acids, short chain CoAs, NAD coenzymes, and other metabolites using liquid chromatography/mass spectrometry