Histology
Overview
The Histology Core Facility provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. The Facility also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. The Facility maintains and provides training for several pieces of common use equipment, such as paraffin microtome, cryostat, and cytospin. Training is also provided for basic histological techniques such as perfusion and tissue collection. Finished slides are returned to the researcher for evaluation, or they may be digitized using our latest addition, the Aperio Scanscope. The Aperio Scanscope allows not only digitizing of slides for archiving, it also offers powerful image analysis tools as well. A tissue acquisition service assists investigators with regulatory/legal compliance, as well as obtaining and storage of human tissue samples and associated clinical data.
Services
Conventional Histology
- Aperio high-resolution slide scanning and optional image analysis for bright field and fluorescence samples
- Routine and custom sectioning
- Paraffin and frozen samples
- Standard and special stain (H&E, PAS, silver impregnation)
Immunohistochemistry
- Double labeling of antibodies
- Immunofluorescence
- Development of custom protocols (overlay assays, competition assays)
- Titration and staining of commercial and custom antibodies
Equipment & Resources
- Aperio Scanscope AT2 and FL systems
- Leica 3050 S cryostat
- Leica RM 2125 paraffin microtome
- Leica BOND-RX automated system for IHC/ISH
- Leica Autostainer ST5010 for H&E
- Shandon Cytospin 3
- Sakura Tissue Tek vacuum infiltration tissue processor
- Leica EG 1160 paraffin embedding station
- MMI Cell Cut Laser Microdissection system
Price List
For a complete list of services, please call (858) 646-3100 ext. 3552 or email us.
| Histology Services | Internal Subsidized | Internal | External Non-Profit | External For-Profit | |
|---|---|---|---|---|---|
| Small Combo/Embed/H&E/5unstained | each | $18 | $22.50 | $24.30 | $47.34 |
| Large Combo/Embed/H&E/10unstained | each | $28 | $35 | $37.80 | $73.64 |
| Additional Sections/Paraffin | per slide | $5.50 | $6.88 | $7.43 | $14.47 |
| Embedding Only/Paraffin | each | $6.50 | $8.13 | $8.78 | $14.47 |
| Immuno Staining (IHC or IF) | per slide | $35 | $43.75 | $47.25 | $92.05 |
| Aperio 20X scan | per slide | $10 | $12.50 | $13.50 | $26.30 |
| Aperio 40X scan | per slide | $20 | $25 | $27 | $52.60 |
| Aperio Fluorescence Scan | per slide | $20 | $25 | $27 | $52.60 |
| Common Cryostat | per hour | $22.20 | $27.60 | $30 | $58.20 |
Contact
Guillermina (Gia) Garcia
Gia has been with Histology core for the past 8 years. She is responsible for the handling and processing of research samples on a day to day basis. She can answer any questions that you may have about your project. Gia is also skilled in a wide range of immunohistochemistry protocols, as well as a variety of histological staining methods.
Please call (858) 646-3100 ext. 3556 or use the button below to send us an email.