Exploratory Pharmacology
Overview
ADME/PK assays enable informed decision making in drug discovery and development. At Sanford Burnham Prebys, the ADME/PK core is focused on developing and running in vitro and in vivo assays to investigate various absorption, digestion, metabolism and excretion parameters of molecules. Our aim is to deliver quality data in a short time to enable medicinal chemists to effectively develop structure-activity relationships and improve drug-like properties in drug discovery and development programs. This facility is integral part of Conrad Prebys Center for Chemical Genomics and works in collaboration with other core facilities across the organization.
ADME/PK core also provides support for grants in terms of planning and budgeting ADME/PK activities, scientific input on drug discovery projects, performing DMPK experiments, and fulfilling reporting requirements. We also provide consultations on standalone service in experimental design, experimentation and reporting DMPK experiments.
Services
Assays
- Stability
Liver microsome, hepatocyte, plasma and formulation stability - Solubility
Kinetic and thermodynamic solubility - Free fraction determination
Plasma protein binding and brain fractionation - PK and bioavailability studies
IV, IP, PO, SC, topical etc. - Dose predictions
Clearance, Brain weight and Maximum Lifespan Potential based First-in-Human predictions - PD and efficacy studies
In vivo models for assessing target engagement, functional activity and assessment of drug like properties
Equipment & Resources
Shimadzu UPLCs
Sciex QTRAP 6500+
Brings about significant enhancement in our bioanalysis capabilities
- Linearity: Triple quadrupole has one of the best range of linearity with MRM3 mode (up to 6 orders of magnitude)
- Sensitivity: LLOQ in pg/mL to fg/ml ranges (results vary for analytes; Attograms sensitivity reported for some analytes)
- Agility: Polarity switching (1millisecond)
- Versatility: Mass range of m/z 5 – 2,000
- Robustness: Multi-day consecutive injection feasible
- Efficiency: Increased ionization efficiency and heat transfer with the new IonDrive Turbo V source
Sciex API 4000
For ADME and bioanalysis
- Linearity: Triple quadrupole has one of the best range of linearity with MRM3 mode (up to 6 orders of magnitude)
- Sensitivity: LLOQ in ng/mL ranges (results vary for analytes; picograms sensitivity reported for some analytes)
- Agility: Polarity switching
- Versatility: Mass range of m/z 5 – 1,000
- Robustness: Multi-day consecutive injection feasible
- Efficiency: Increased ionization efficiency and heat transfer with the new IonDrive Turbo V source
Price List
For a Price List, please email us.
Leadership
Raghuveer Ramachandra, PhD
Associate Director