Technology supporting the three programs
The 10 shared resources of our Cancer Center are specialized service facilities that support the cancer research efforts of our members, as well as other nonprofit and for-profit investigators in cancer research.
- Animal Facility
Vivarium with full-service husbandry (colony management, breeding, weaning, tail samples), and procedures, including injections, bleeds, tumor measurements, and surgeries
- Animal Imaging & Analysis
Live animal imaging utilizing luminescence, fluorescence, ultrasound, and X-rays. Hematology (CBC) and blood chemistry analysis
- Tumor Analysis
Supports xenograft tumor studies including PDX, testing of anti-tumor compound efficacy, deriving cell cultures from PDX models for HTS analysis
Cell Imaging and Histology
- Cell Imaging
11 microscopes: Nikon super resolution/confocal system, three additional confocal systems (including multi-photon), bright field, fluorescence, FRET, laser TIRF, live cell imaging. Microscopes integrated with image capture systems and advanced image analysis software.
Fixed/frozen sections, automated staining for H&E and IHC, laser capture, high resolution slide scanning (bright field and fluorescence), assistance with tissue acquisition, including TMAs
- Nuclear Magnetic Resonance
Infrastructure includes, two 600 MHz instruments with cryoprobes, a 500 MHz instrument with wide bore probe for solid state analysis, and a highly automated 400 MHz instrument supporting medicinal chemistry.
Robotics and imaging incubators for crystallization trials, X-ray generator with 2 detectors
- Protein Analysis
Includes micro-ITC, microscale thermophoresis, DSC, and Analytical Ultracentrifugation
- Flow Cytometry
Core staff operate two BD cell sorters with up to 16 colors, which are housed in biosafety enclosures. There are also five analytical cytometers with up to 18 colors (three equipped with plate loaders), and an Amnis imaging flow cytometer
Next-gen sequencing analysis including library prep, sequencing (Illumina NextSeq 500), single-cell sequencing (10X and Bio-Rad ddSeq), and data analysis. Full-service Q-PCR, NanoString nCounter, STR cell line verification, shared analytical equipment
Analysis from simple protein IDs to characterizing proteome-wide PTMs. Automated sample prep, advanced Thermo LC-MS systems include two Fusion Lumos Tribrids, Velos Elite, QExactive plus, and Quantiva. Hardware and software for proteomics data analysis.
Informatics, statistical, and pathway analysis of large ‘omics datasets, data mining, systems biology, investigator training. Dedicated HPC cluster and data storage, shared tools including Qiagen Omicsoft Suite, Ingenuity Pathways, NextBio/Illumina Correlation Engine, GeneGo Metacore
- Functional Genomics
siRNA/shRNA/miRNA/cDNA/CRISPR libraries, HTS assay development and screening, hit follow-up, CRISPR-engineered cell lines, production of viral vectors (lentivirus, retrovirus, adenovirus, AAV)
Chemical Library Screening (part of the Prebys Center)
- Assay Development
Assists in designing, developing, and and implementing HTS assays in 384/1536 well formats, utilizing a wide variety of assay types, and also supports follow-up SAR studies
- Medicinal Chemistry
Custom compound synthesis, library design/synthesis, guidance in hit to lead
- Chemical Library & High-Throughput Screening
Broad and focused libraries of compounds, including cancer-targeted drugs/compounds. Biochemical and cell-based HTS utilizing extensive robotics and assay instrumentation
- High Content Screening
Develops, performs and analyzes high throughput microscopy-based assays on fixed or live cells, utilizing a PE Opera Phenix with integrated robotics or several other HCS systems